human vcl Search Results


human vcl  (Genecopoeia)
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Genecopoeia human vcl
Fig. 5. miR-142-3p directly regulate genes involved in cell migration, phagocytosis and cell polarity. (A–C; Upper panel) Sequence alignment of predicted miR-142- 3p binding sites in the 3′UTR of <t>VCL,</t> Dab2 and Skap2. Only the binding sites with mfe < −20 kcal/mol are shown. (A–C; Lower panel) HEK293 cells were co- transfected with dual luciferase <t>reporter</t> <t>plasmids</t> containing 3′UTR of VCL, Dab2 and Skap2 or control vector and miR-142-3p or control mimic. After 36 h, cell lysates were prepared to measure renilla and firefly luciferase activity. Renilla activity was normalized to firefly activity and the ratios were subsequently normalized to empty vector transfected with miR-142-3p mimic set as 1. Data are expressed as ± SEM of four independent transfections. Student's t-test was conducted to calculate p-values. *p < 0.05, **p < 0.01, ***p < 0.001. (D) Expression of VCL, Dab2 and Skap2 in MΦ by western blot. Day 7 differentiated cells were transfected with miR-142-3p mimic or control mimic. Cell lysates were prepared after 36 h of transfection and VCL, Dab2 and Skap2 levels were detected by immunoblotting. The expression level of GAPDH was included as loading control. (E) The corresponding densitometric analysis by Image Lab software is also shown. Data are means ± SEM of three independent experiments (*p < 0.05, **p < 0.01; compared with the control mimic).
Human Vcl, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vinculin elisa kit  (Shanghai Korain Biotech Co Ltd)
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Shanghai Korain Biotech Co Ltd vinculin elisa kit
Fig. 5. miR-142-3p directly regulate genes involved in cell migration, phagocytosis and cell polarity. (A–C; Upper panel) Sequence alignment of predicted miR-142- 3p binding sites in the 3′UTR of <t>VCL,</t> Dab2 and Skap2. Only the binding sites with mfe < −20 kcal/mol are shown. (A–C; Lower panel) HEK293 cells were co- transfected with dual luciferase <t>reporter</t> <t>plasmids</t> containing 3′UTR of VCL, Dab2 and Skap2 or control vector and miR-142-3p or control mimic. After 36 h, cell lysates were prepared to measure renilla and firefly luciferase activity. Renilla activity was normalized to firefly activity and the ratios were subsequently normalized to empty vector transfected with miR-142-3p mimic set as 1. Data are expressed as ± SEM of four independent transfections. Student's t-test was conducted to calculate p-values. *p < 0.05, **p < 0.01, ***p < 0.001. (D) Expression of VCL, Dab2 and Skap2 in MΦ by western blot. Day 7 differentiated cells were transfected with miR-142-3p mimic or control mimic. Cell lysates were prepared after 36 h of transfection and VCL, Dab2 and Skap2 levels were detected by immunoblotting. The expression level of GAPDH was included as loading control. (E) The corresponding densitometric analysis by Image Lab software is also shown. Data are means ± SEM of three independent experiments (*p < 0.05, **p < 0.01; compared with the control mimic).
Vinculin Elisa Kit, supplied by Shanghai Korain Biotech Co Ltd, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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e cadherin  (Cusabio)
93
Cusabio e cadherin
Fig. 5. miR-142-3p directly regulate genes involved in cell migration, phagocytosis and cell polarity. (A–C; Upper panel) Sequence alignment of predicted miR-142- 3p binding sites in the 3′UTR of <t>VCL,</t> Dab2 and Skap2. Only the binding sites with mfe < −20 kcal/mol are shown. (A–C; Lower panel) HEK293 cells were co- transfected with dual luciferase <t>reporter</t> <t>plasmids</t> containing 3′UTR of VCL, Dab2 and Skap2 or control vector and miR-142-3p or control mimic. After 36 h, cell lysates were prepared to measure renilla and firefly luciferase activity. Renilla activity was normalized to firefly activity and the ratios were subsequently normalized to empty vector transfected with miR-142-3p mimic set as 1. Data are expressed as ± SEM of four independent transfections. Student's t-test was conducted to calculate p-values. *p < 0.05, **p < 0.01, ***p < 0.001. (D) Expression of VCL, Dab2 and Skap2 in MΦ by western blot. Day 7 differentiated cells were transfected with miR-142-3p mimic or control mimic. Cell lysates were prepared after 36 h of transfection and VCL, Dab2 and Skap2 levels were detected by immunoblotting. The expression level of GAPDH was included as loading control. (E) The corresponding densitometric analysis by Image Lab software is also shown. Data are means ± SEM of three independent experiments (*p < 0.05, **p < 0.01; compared with the control mimic).
E Cadherin, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human vcl (vinculin) elisa kit  (Elabscience Biotechnology)
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Elabscience Biotechnology human vcl (vinculin) elisa kit
Serum <t>vinculin</t> in the studied groups.
Human Vcl (Vinculin) Elisa Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vinculin (vcl) (nm_003373) human untagged clone  (OriGene)
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Serum <t>vinculin</t> in the studied groups.
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mirna 3´utr target expression clone for human vcl  (Genecopoeia)
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Genecopoeia mirna 3´utr target expression clone for human vcl
Serum <t>vinculin</t> in the studied groups.
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vinculin (vcl) (nm_003373) human tagged orf clone  (OriGene)
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Serum <t>vinculin</t> in the studied groups.
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primary rabbit anti human vcl antibodies  (ZenBio)
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Serum <t>vinculin</t> in the studied groups.
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vinculin (vcl) (nm_003373) human recombinant protein  (OriGene)
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Serum <t>vinculin</t> in the studied groups.
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primary rabbit anti-human vcl antibodies 380393  (ZenBio)
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Serum <t>vinculin</t> in the studied groups.
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vinculin vcl nm 003373 human tagged orf clone  (OriGene)
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VCL GFP tagged Human vinculin VCL transcript variant 2
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human vcl primer pair 6 (ooca01811)  (Aviva Systems)
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Human VCL Primer Pair 6 for RT-PCR (reverse transcription followed by polymerase chain reaction) analysis of mRNA expression.; Human VCL Primer Pair 6
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Image Search Results


Fig. 5. miR-142-3p directly regulate genes involved in cell migration, phagocytosis and cell polarity. (A–C; Upper panel) Sequence alignment of predicted miR-142- 3p binding sites in the 3′UTR of VCL, Dab2 and Skap2. Only the binding sites with mfe < −20 kcal/mol are shown. (A–C; Lower panel) HEK293 cells were co- transfected with dual luciferase reporter plasmids containing 3′UTR of VCL, Dab2 and Skap2 or control vector and miR-142-3p or control mimic. After 36 h, cell lysates were prepared to measure renilla and firefly luciferase activity. Renilla activity was normalized to firefly activity and the ratios were subsequently normalized to empty vector transfected with miR-142-3p mimic set as 1. Data are expressed as ± SEM of four independent transfections. Student's t-test was conducted to calculate p-values. *p < 0.05, **p < 0.01, ***p < 0.001. (D) Expression of VCL, Dab2 and Skap2 in MΦ by western blot. Day 7 differentiated cells were transfected with miR-142-3p mimic or control mimic. Cell lysates were prepared after 36 h of transfection and VCL, Dab2 and Skap2 levels were detected by immunoblotting. The expression level of GAPDH was included as loading control. (E) The corresponding densitometric analysis by Image Lab software is also shown. Data are means ± SEM of three independent experiments (*p < 0.05, **p < 0.01; compared with the control mimic).

Journal: Biochimica et biophysica acta. Gene regulatory mechanisms

Article Title: Impaired cell migration and structural defects in myeloid cells overexpressing miR-30b and miR-142-3p.

doi: 10.1016/j.bbagrm.2020.194628

Figure Lengend Snippet: Fig. 5. miR-142-3p directly regulate genes involved in cell migration, phagocytosis and cell polarity. (A–C; Upper panel) Sequence alignment of predicted miR-142- 3p binding sites in the 3′UTR of VCL, Dab2 and Skap2. Only the binding sites with mfe < −20 kcal/mol are shown. (A–C; Lower panel) HEK293 cells were co- transfected with dual luciferase reporter plasmids containing 3′UTR of VCL, Dab2 and Skap2 or control vector and miR-142-3p or control mimic. After 36 h, cell lysates were prepared to measure renilla and firefly luciferase activity. Renilla activity was normalized to firefly activity and the ratios were subsequently normalized to empty vector transfected with miR-142-3p mimic set as 1. Data are expressed as ± SEM of four independent transfections. Student's t-test was conducted to calculate p-values. *p < 0.05, **p < 0.01, ***p < 0.001. (D) Expression of VCL, Dab2 and Skap2 in MΦ by western blot. Day 7 differentiated cells were transfected with miR-142-3p mimic or control mimic. Cell lysates were prepared after 36 h of transfection and VCL, Dab2 and Skap2 levels were detected by immunoblotting. The expression level of GAPDH was included as loading control. (E) The corresponding densitometric analysis by Image Lab software is also shown. Data are means ± SEM of three independent experiments (*p < 0.05, **p < 0.01; compared with the control mimic).

Article Snippet: All transfections were performed in quadruplicate using 0.5 μL Lipofectamine 2000 (Invitrogen), 120 ng dual luciferase reporter control (CmiT000001-MT06 miRNA Target clone control vector for pEZX-MT06) or plasmids containing human VCL (NM_003373.3; HmiT018467-MT06), human DAB2 (NM_001244871.1; HmiT055467-MT06) or human SKAP2 (NM_001303468.1; HmiT067400-MT06; All from GeneCopoeia Incorporated, Rockville, MD, USA) were co-transfected with a final concentration of 1 pmol, 5 pmol and 10 pmol of synthetic miR-142-3p or control mimics (10 pmol) (Qiagen).

Techniques: Migration, Sequencing, Binding Assay, Transfection, Luciferase, Control, Plasmid Preparation, Activity Assay, Expressing, Western Blot, Software

Serum vinculin in the studied groups.

Journal: Biologics : Targets & Therapy

Article Title: Elevated Serum Vinculin in Patients with HBV/HCV-Associated Liver Cirrhosis and Hepatocellular Carcinoma: A Pilot Study

doi: 10.2147/BTT.S405500

Figure Lengend Snippet: Serum vinculin in the studied groups.

Article Snippet: Serum VCL level was assayed using Elabscience Human VCL (Vinculin) ELISA Kit, Cta No: E-EL-H1795, Elabscience Biotechnology Inc. USA.

Techniques:

Correlation Between Serum Vinculin (VCL) Level with the Laboratory Parameters in the HCC Group (n = 51)

Journal: Biologics : Targets & Therapy

Article Title: Elevated Serum Vinculin in Patients with HBV/HCV-Associated Liver Cirrhosis and Hepatocellular Carcinoma: A Pilot Study

doi: 10.2147/BTT.S405500

Figure Lengend Snippet: Correlation Between Serum Vinculin (VCL) Level with the Laboratory Parameters in the HCC Group (n = 51)

Article Snippet: Serum VCL level was assayed using Elabscience Human VCL (Vinculin) ELISA Kit, Cta No: E-EL-H1795, Elabscience Biotechnology Inc. USA.

Techniques: